Fig. 1: Overview of the study.

a Benchmarking label-free quantification by data-dependent acquisition (LFQ-DDA) and data-independent acquisition (LFQ-DIA), as well as isobaric tandem mass tag (TMT)-based quantification, on simulated microbial communities consisting of 12 species of bacteria and feces samples spiked with 6 species. b Profiling human gut metaproteome in clinical samples of pancreatic cancer patients and mild cognitive impairment patients.