Fig. 1: CORT induces depression and altered composition of fecal microbiota in mice. | npj Biofilms and Microbiomes

Fig. 1: CORT induces depression and altered composition of fecal microbiota in mice.

From: Gut microbiota dysbiosis-mediated ceramides elevation contributes to corticosterone-induced depression by impairing mitochondrial function

Fig. 1

a Experimental design and the timeline. b Comparison of the immobility time in the TST for the two groups of mice. c Comparison of the immobility time in the FST for the two groups of mice. d Representative images of EdU staining within the DG of hippocampus. e Quantitative analysis of EdU+ cell within the DG of the hippocampus. f Beta-diversity-based principal component analysis (PCA) plots showing microbial compositional difference at genus level between the two groups. g Alpha diversity analysis of fecal microbial community diversity (Sobs index). h Bar graph showing the relative bacterial abundance at the order level. i Bar graph showing the relative bacterial abundance at the genus level. j Bar graph showing the relative bacterial abundance at the species level. For behavioral tests, n = 24 for each group, comparisons were performed using Student’s t test. For the EdU test, n = 5 for each group, comparisons were performed using nonparametric Mann–Whitney U test. For 16S rRNA sequencing, the Veh-treated group: n = 8; CORT-treated group: n = 12; comparisons were performed using Welch’s t test. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. CORT corticosterone, Veh vehicle, TST tail suspension test, FST forced swimming test, FC fecal collection, EdU-I EdU injection, DG dentate gyrus. Scale bar: 100 µm. Sobs: total number of species observed in a sample.

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