Fig. 4: E-cigarettes induce quorum-sensing regulated gene expression in oral biofilms.
From: Toxic cultures: e-cigarettes and the oral microbial exposome
Heatmap of KEGG orthologs that were upregulated following exposure to nicotine-free or nicotine plus vapor or clean-air is shown in (A). All samples were run in triplicates, and the experiments were duplicated. Data supporting (A) can be found in Supplemental Table 4. Co-occurrence networks between metabolites and microbial transcripts in each group are shown in (B–G). Commensal-rich biofilms are shown in (B, E), Intermediate in (C, F), and pathogen-rich in (D, G). Nicotine-free vapor exposure is represented in (B–D) and nicotine-plus vapor exposure is shown in (E–G). Each network graph contains nodes (circles) and edges (lines). Nodes represent metabolites (pink) and KEGG-annotated transcripts (blue), and edges represent Spearman’s rho. Edges are colored green for positive correlation and red for negative correlation. Only significant correlations (P < 0.05, t test) with a coefficient of at least 0.80 are shown.
