Table 1 Statistics for WGBS-based DNAm profiles

Subjects

Males, N (%)

48 (47.1)

49 (48.0)

Age, year^a

58.5 ± 11.0

58.0 ± 11.4

Sequencing statistics

Number of raw reads^a

780,709,034 ± 45,934,514

779,212,752  ± 40,833,955

Number of raw bases^a

97,588,629,235 ± 5,741,814,291

97,401,593,968  ± 5,104,244,342

Raw depth^a

31.1 ± 1.8

31.0 ± 1.6

Number of reads after quality-control filtering^a

624,432,868 ± 38,766,158

667,934,331 ± 33,002,407

Number of bases after quality-control filtering^a

47,245,324,384 ± 3,327,997,912

52,472,000,722 ± 2,376,329,508

Depth after quality-control filtering^a

15.1 ± 1.1

16.7 ± 0.8

CpG statistics

Number of autosomal CpGs in the human genome

26,752,702

26,752,702

Number of autosomal CpGs covered by at least 1 read

24,932,694 ± 163,384

24,939,224 ± 121,488

Percentage of autosomal CpGs covered by at least 1 read

93.2 ± 0.6

93.2 ± 0.5

Number of autosomal CpGs after depth filtering

23,404,723 ± 362,243

23,584,230 ± 238,187

Percentage of autosomal CpGs after depth filtering

87.5 ± 1.4

88.2 ± 0.9

Number of autosomal CpGs with a call rate of ≥ 50%

23,941,843

24,037,541

Percentage of autosomal CpGs with a call rate of ≥ 50%

89.5

89.9