Fig. 3: Longitudinal immune evaluation.
From: Pre-T cell receptor-α immunodeficiency detected exclusively using whole genome sequencing
A Total CD3+ cells, (B) CD3+CD4+ cells and (C) CD3+CD8+ cells remained low since birth, while (D) CD19+ B cells, (E) CD16+CD56+ NK cells, and (F) neutrophils were within normal range. G Monocytes were within low to normal ranges. Gray bars represent age-matched reference ranges. H upper Flow analysis of TCRVβ repertoire at age 1 month revealed CD4 Vβ 2, 7, 13.6, and 23 were under-represented while Vβ 5.1, 12, 20, and 22 were over-represented (upper). H lower CD8 Vβ 2, 9, 13.2, 13.6, and 21.3 were under represented while Vβ 5.1, 20, and 22 were over-represented (lower). I Profiling of the T cell receptor β variable (TRBV) genes by sequencing analysis at age 28 months revealed similar gene usage in the patient and control.
