Fig. 2: Activity-dependent αS phosphorylation is S129-specific and reversible.
From: Dynamic physiological α-synuclein S129 phosphorylation is driven by neuronal activity
a–d DIV17-21 rat cortical neurons treated with 20 µM PTX for 2 h. WB for total αS and indicated phospho-sites. N = 6 (a–c) or 4 (d) independent experiments on different days. ****p < 0.0001; ns not significant. Mean +/− SD. Paired t-tests; two-tailed. e Reversible pS129 triggered by neuronal activity in DIV17-21 rat cortical neurons as indicated in the schematic (20 µM PTX, 1 µM TTX). WB for total αS and pS129. N = 4 independent experiments on different days, n = 16 biological replicates total; ****p < 0.0001. Mean +/− SD. Brown-Forsythe and Welch ANOVA with Dunnett’s T3 post hoc test for multiple comparisons. f Kinetics of reversible pS129 in DIV17-21 rat cortical neurons as indicated in the schematic (20 µM PTX, 1 µM TTX). WB for total αS and pS129. N = 5 independent experiments on different days, n = 15 biological replicates total; *p < 0.1; ***p < 0.001. Mean +/− SD. Brown-Forsythe and Welch ANOVA with Dunnett’s T3 post hoc test for multiple comparisons.
