Fig. 6: Transmission electron microscopy analysis in the SNpc using double immunogold labeling with CAB101 (anti-RgpB; 6 nm gold particles, arrows) or CAB102.1 (anti-Kgp; 6 nm gold particles, arrows), and anti-αSynp (15 nm gold particles, arrowheads).

a−c Kgp and αSynp in association with αSynp plaques. a An overview image of a neuromelanin-positive cell with multiple circular αSynp plaques (P). b Enlarged region from (a). αSynp immunogold labeling (black arrowheads) can be seen on the periphery of the plaques and in proximity to neuromelanin (NM) granules. c Enlarged region from (a). αSynp (black arrowheads) and Kgp (black arrows) immunogold labeling can be seen on medium electron-dense tendrils at the periphery of the plaque. d A control case with a 15 nm gold particle (black arrowhead) marking the rare presence of αSynp in the cytoplasm of a neuromelanin cell. e More frequent labeling of αSynp was observed in PD cases (black arrowheads). f Kgp (black arrows) associated with αSynp (black arrowhead) in cytoplasm near neuromelanin (NM) and mitochondria (m) (g). g Kgp (black arrows) and αSynp (black arrowhead) in proximity and within a mitochondrion (m). h, h’ Kgp (black arrows) associated with αSynp (black arrowhead) in a mitochondrion (m). i Kgp (black arrow) associated with αSynp (black arrowhead) in a mitochondrion (m). j, j’ RgpB (black arrows) associated with αSynp (black arrowhead) in a mitochondrion (m). k, k’ RgpB (black arrow) associated with αSynp (black arrowhead) in a mitochondrion (m). l, l’ Rgp (black arrows) associated with αSynp (black arrowhead) on the surface of a neuromelanin granule. NM, neuromelanin; m, mitochondria, P, αSynp plaque. Scale bars: 5 µm (a), 1 µm (b, d), 500 nm (e, f, g, h, j, k, l, m), 200 nm (c, i).