Table 3 IF: immunofluorescence quantification, IHC: immunohistochemistry, 3D: three-dimensional visualization, TEM: transmission electron microscopy
Antibody/chemicals/detection | Source | Identifier | Dilution |
|---|---|---|---|
CAB101 RgpB, Rabbit (0.5 mg/ml) | Cortexyme, Inc. | IHC; 1:1000 IF, 3D, TEM; 1:500 | |
CAB102.1 Kgp, Rabbit (0.46 mg/ml) | Cortexyme, Inc. | IHC; 1:1000 IF, 3D, TEM; 1:500 | |
Anti-Alpha-synuclein (phosphoS129) antibody [P-syn/81 A], Mouse monoclonal | Abcam | Cat# ab184674, RRID:AB_2819037 | IF, 3D, TEM; 1:3,000 |
Anti-NeuN purified, Guinea Pig polyclonal | Millipore | Cat# ABN90P, RRID:AB_2341095 | IF; 1:250 |
biotinylated Goat anti-Rabbit | Thermo Fisher Scientific | Cat# A-16100 | IHC; 1:1000 |
biotinylated Donkey anti-Rabbit | Thermo Fisher Scientific | Cat# A-16027 | IHC; 1:1000 |
Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11034, RRID:AB_2576217 | IF, 3D; 1:250 |
Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 | Thermo Fisher Scientific | Cat# A-11032, RRID:AB_2534091 | IF, 3D; 1:250 |
Goat Anti-Guinea Pig IgG (H + L) Highly Cross-adsorbed Antibody, Alexa Fluor 647 Conjugated | Molecular Probes | Cat# A-21450, RRID:AB_141882 | IF, 3D; 1:250 |
Hoechst 33342, Trihydrochloride, Trihydrate | Invitrogen Molecular Probes | Cat# H1399 | IF, 3D; 1:20,000 |
ExtrAvidin−Peroxidase buffered aqueous solution | Sigma-Aldrich | Cat#E2886-1ML | IHC; 1:1000 |
Goat anti-Rabbit IgG (H + L) coupled with 6 nm gold, EM grade | Electron Microscopy Science, Auron 106.011 | Cat# 25103 | TEM; 1:50 |
Goat anti-Mouse IgG (H + L) coupled with 15 nm gold, EM grade | Electron Microscopy Science, Auron 115.022 | Cat# 25132 | TEM; 1:50 |
