Fig. 4: Inhibition of NRF2 via AEM1 treatment induces HepaRG differentiation. | npj Regenerative Medicine

Fig. 4: Inhibition of NRF2 via AEM1 treatment induces HepaRG differentiation.

From: Inhibition of nuclear factor (erythroid-derived 2)-like 2 promotes hepatic progenitor cell activation and differentiation

Fig. 4

a mRNA expression of genes characteristic of mature hepatocytes (left) or of indifferentiated cells (right) in untreated or AEM1-treated HepaRG cells after 2 weeks. Data in the graph are represented as mean ± SD of three independent experiments. Statistical differences were assessed by student’s t test. *p < 0.05 vs untreated; **p < 0.01 vs untreated; ***p < 0.001 vs untreated. b Flow cytometry phenotype of untreated or AEM1-treated HepaRG cells after 2 weeks. c Representative recordings of oxygen consumption rate (nmolO2/min/mln cell) in untreated or AEM1-treated HepaRG cells after 2 weeks. d Mitochondrial membrane potential measured in untreated or AEM1-treated HepaRG cells after 2 weeks via the JC-10 assay.

Back to article page