Fig. 2: Purified exosome product functional characterization and in vitro human skeletal muscle myoblasts (HSMM) culturing with increasing concentration of PEP. | npj Regenerative Medicine

Fig. 2: Purified exosome product functional characterization and in vitro human skeletal muscle myoblasts (HSMM) culturing with increasing concentration of PEP.

From: Exosome biopotentiated hydrogel restores damaged skeletal muscle in a porcine model of stress urinary incontinence

Fig. 2

A IncuCyte proliferation, B chemotaxis, and C wound scratch analyses of HSMM grown with increasing concentration of PEP ranging from 1.25 × 1011 exosomes/mL to 5 × 1011 exosomes/mL and media supplemented with 10% fetal bovine serum (FBS) versus serum-free media. D–I Representative immunocytochemistry of a 96 h time-course of HSMM cultured with serum-free media (Control) or serum-free media plus 2.5 × 1011 exosomes/mL PEP (PEP). Immunostaining for MyoD (D; green, scale = 100 µm), Pax7 (F; red, scale = 100 µm), and Myosin Heavy Chain (MHC, H; green, scale = 20 µm). Nuclei counterstained with DAPI (blue). ImageJ blind quantification of E MyoD+ Area/DAPI + Area, G Pax7+ Area/DAPI + Area, and I Myosin Heavy Chain+ Area/DAPI object count, relative to day zero. N.S. Not significant; *p < 0.05; **p < 0.005. Data represents AVE ± SEM. Student’s t-test was utilized.

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