Fig. 2: Murine neonatal cardiac B cells possess the greater ability to inhibit inflammatory responses and promote angiogenesis after cardiac injury.
From: Murine neonatal cardiac B cells promote cardiomyocyte proliferation and heart regeneration
a Representative immunofluorescent staining images and quantification of EdU+ cardiomyocytes from myocardial tissues of adult CD19DTR mice with DT or PBS administration at day 7 post-MI. Each symbol in quantification indicates one representative image from one heart section of one mouse (MI + PBS group, n = 7 mice; MI + DT group, n = 9 mice; mean ± SD; unpaired Student’s t-test). Scale bars, 20 μm. b Violin plots showing the expression scores of cell proliferation-associated genes (Slpi, Ighg1, Lcn2, S100a8, S100a9, and Cxcl2) in cardiac B cells from adult mice subjected to MI or sham operation (GSE163465). c GO enrichment analysis of DEGs in B cells from heart tissues at day 7 after AR operated on P1 and P7 mice. d Heatmap of DEGs enriched in angiogenesis, inflammatory responses, and chemokine signaling pathway of cardiac B cells prepared as in c and from adult mouse heart after MI (GSE163465). e Heatmap of top DEGs from cardiac B cells prepared as in c and from adult mouse heart after MI (GSE163465). f UMAP plots displaying B cell clusters in heart tissues prepared as in c and from adult mouse heart after MI (GSE163465). g Distribution of B cell proportions in each of the nine clusters from heart tissues prepared as in c and from adult mouse heart after MI (GSE163465). **p < 0.01.
