Fig. 4: The nbn mutant exhibits normal cell proliferation but aberrant cell apoptosis. | npj Regenerative Medicine

Fig. 4: The nbn mutant exhibits normal cell proliferation but aberrant cell apoptosis.

From: The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway

Fig. 4

a Confocal z-stack projection images showing the label of EdU (red), Dendra2 (green), and DAPI (blue) in regenerating livers at R24h and R48h in wild-type and nbn mutant. b Quantification of the percentage of EdU+ cells among Dendra2+ cells, n = 7 larvae per group. c Confocal z-stack projection images showing the label of TUNEL (red), Dendra2 (green), and DAPI (blue) in regenerating livers at R24h and R48h. d Quantification of the percentage of TUNEL+ cells among Dendra2+ cells, n = 7 larvae per group. Asterisks indicate statistical significance: ****P < 0.0001 using t-tests analysis when compared to control. Scale bars, 100 μm; error bars, ±SEM. R regeneration time after the withdrawal of Mtz, WT wild-type, DAPI 4′, 6-diamidino-2-phenylindole, n.s. no significance.

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