Fig. 4: Human donor-derived myofibers engrafted in dystrophic mice are also more transcriptionally mature compared to in vitro-differentiated myotubes.
From: The adult environment promotes the transcriptional maturation of human iPSC-derived muscle grafts
a Heatmap displaying expression values (logCPM) for all DEGs between human in vitro myotubes and in vivo myofibers in NSG-mdx4Cv mice (“DMD in vivo myofibers”). Hierarchical clustering was performed to group samples and genes by similarity, which identified five unique clusters of genes arose. High expression in orange, low expression in teal. b Tables describing all muscle-related pathways enriched in Cluster 2 and all significant pathways enriched in Cluster 4 after pathway analysis (DAVID, GOTERM Biological Processes). c, d Bar graphs showing the log-fold change of genes that are annotated to two different gene sets that emerged as top pathways. Enriched genes in orange and downregulated genes in teal. e Heatmap of expression (logCPM) for major myogenic genes for in vitro myotubes with DMD in vivo myofibers (left). Gray bars on the left side highlight the development stage associated with each gene (1 - myogenic commitment, 2 – differentiation, 3 – immature myofiber, 4 – adult myofiber). Heatmap of expression (logCPM) for other muscle-related genes involved in skeletal muscle structure, comparing in vitro myotubes with DMD in vivo myofibers (right). High expression is in orange and low expression in teal, where color is based on row minimums/maximums. Light blue shading represents instances where no human transcripts were measured for that sample (n = 2 and n = 3 for in vitro myotube and DMD in vivo myofiber samples, respectively).
