Fig. 1: Experimental design and quality control of ribo-seq data.

a Singly caged male C57BL/6JRj mice were maintained from 15 to 30 months of age in either standard laboratory conditions or treatment with RM or 35%CR and compared with 10-month-old adult mice. The Quadriceps femoris muscle was analyzed by both RNA-seq and ribo-seq. Mouse figure was created with BioRender.com. b Length distribution of ribo-seq reads from all sequenced samples. c The offset of P-sites in reads of individual lengths (in the range of 11–13 nts) was first determined, and then all reads were registered using these offsets. The proportion of reads associated with each of the 3 reading frames was then calculated. Each dot represents one sample, the height of the bar is the average over samples, and the standard deviations are also indicated. Two-sided Student’s t-test was used to calculate p-values. d Relative proportion of reads in a sample mapping to 5′UTR, CDS or 3′UTR regions of the mRNAs. Each dot corresponds to a sample, the height of the bars represents the average over samples, and standard deviations are also indicated.