Fig. 4: Comparative characterization of S6w1 scRNAseq dataset with Azimuth human pancreas reference and donor islets.

a Azimuth UMAP- base embedding projection to identify human pancreatic islet cell populations clustered from S6w1 scRNAseq datasets (n = 2 batches from a healthy iPSC line, 17,393 cells) after annotating with reference human pancreas dataset- Satija lab https://azimuth.hubmapconsortium.org/references/human_pancreas/ (GSE217837; n = 3 donors, 35,289 cells). b UMAP projection representing total 9 clusters identified between S6w1 SC-islets and donor adult islets using RStudio v2023.06.2 + 561 and Seurat v4.3.2. c Feature plots representing INS and GCG expression in identified clusters between S6w1 SC-islets and adult islets. d Volcano plot to show differentially expressed genes in S6w1 islet dataset in comparison to donor islets. Genes in red show upregulated genes and in cyan downregulated genes. e Violin plots to show comparative gene expression for key β cell specific markers between S6w1 islets and adult islets. Expression values on y-axis are shown as z-score normalized data for each gene. f Feature dot plots displaying representative markers for SC- β, -α, -δ, and -γ cells on individual clusters identified between islets and adult islets. Red color dots denote human donor islets and blue corresponds to S6w1 islets clusters. The size of the dots denotes counts expressing these features matched to the scale (0–100%) g Violin plots showing comparative gene expression distribution for INS and PCSK1 in individual cluster between S6w1 islets and donor islets. h Violin plots comparing gene expression levels for insulin secretion and OXPHOS associated genes between S6w1 islets and donor islets. Expression values on y-axis are shown as z-score normalized data for each gene.