Fig. 4: Antigenicity and immunogenicity of unmodified and cross-linked trimers. | npj Vaccines

Fig. 4: Antigenicity and immunogenicity of unmodified and cross-linked trimers.

From: Profound structural conservation of chemically cross-linked HIV-1 envelope glycoprotein experimental vaccine antigens

Fig. 4

a, b Mouse serum IgG responses to immunization with (a) ConM (M) or ConM-EDC (M-EDC), or (b) ConS (S) or ConS-EDC (S-EDC), assayed against unmodified (M or S) or cross-linked (M-EDC or S-EDC) versions of the protein, where n = 8 mice, 4 mice/group from n = 2 pooled independent experiments. **p < 0.01, ****p < 0.001, one-way ANOVA with Sidak’s multiple comparison correction. c, d Mouse splenocyte antigen-specific cytokine release measured by multiplex bead array after homologous unmodified ConM (c) or ConS (d) in vitro restimulation. Data pooled from n = 2 experiments, datum points represent individual mice. *p < 0.05, Mann—Whitney U. e Endpoint ELISA titers of rabbit antiserum at week 12. f, g Neutralization activity against homologous and heterologous ConM (f) and ConS (g) env pseudoviruses represented by the reciprocal serum dilution giving 50% inhibition (IC50). All error bars represent ±1 standard deviation.

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