Fig. 9: CD8+ T-cell subpopulations in the lung during RSV infection in dependency of prior vaccination.

Balb/c mice were immunized and challenged as described in Fig. 6 and lymphocytes from lungs were examined on respective days post-infection. Antigen experienced CD8+ T-cells were identified by CD44+ and F85-93-specific pentamer staining. a The absolute number of CD44+ F85-93+ CD8+ T-cells was summarized for each group. Within the CD44+ F85-93+ CD8+ T-cell population tissue resident memory T-cells (TRM; KLRG1-CD103+CD69+) (b), central memory T-cells (TCM; CD127+KLRG1-CD69-CD103-) (c), effector memory T-cells (TEM; CD127+KLRG1+) (d) and effector T-cells (TEFF; CD127-KLRG1+) (e) were determined. Time points represent mean values with +SEM; all groups per time point n = 5. Data were analysed by two-way ANOVA followed by Dunnett´s multiple comparison test. Statistically significant differences were indicated among naive and vaccinated groups; (o: statistically significant worse than naive; +: statistically significant better than naive).