Fig. 2: FACS-based whole-genome CRISPR/Cas9 screening. | npj Vaccines

Fig. 2: FACS-based whole-genome CRISPR/Cas9 screening.

From: CRISPR/Cas9 screens identify key host factors that enhance rotavirus reverse genetics efficacy and vaccine production

Fig. 2

A Schematic flowchart for RV FACS-based loss-of-function CRISPR screening approach. Image was created using Adobe Illustrator. B Plot of the scores of -Log10(p value) of the top hits in the screen, sorted on the x-axis by gene Rank. C Plot of the scores of Log2(fold change) of the top hits in the screen, sorted on the x-axis by gene Rank. D Wild-type (WT) and indicated genes knockout MA104 cells were infected with rRRV-GFP (MOI = 1), and GFP signal was measured by flow cytometry analysis. E Protein function analysis by Panther Classification System. For D, experiments were repeated at least three times in triplicates. Statistical analysis: ****P < 0.0001 using one-way ANOVA with Tukey’s post hoc test.

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