Fig. 6: Increased vaccine production in TMEM236 knockout MA104 cells and Vero cells. | npj Vaccines

Fig. 6: Increased vaccine production in TMEM236 knockout MA104 cells and Vero cells.

From: CRISPR/Cas9 screens identify key host factors that enhance rotavirus reverse genetics efficacy and vaccine production

Fig. 6

WT and knockout MA104 cells in T25 flask were infected with Rotarix (A) and RotaTeq (B) (MOI = 0.01) in the presence of trypsin (0.5 μg/ml) for 96 h. Viral titers were determined by FFU assays. Data represent the average of three experiments; error bars indicate SEM (one-way ANOVA with Tukey’s post hoc test; ****P < 0.0001). WT, TMEM236 knockout, and complemented (expressing V5-TMEM236 in TMEM236 knockout cells) Vero cells in T25 flask were infected with Rotarix (C), RotaTeq (D), rVSV-ZEBOV (E), and RotaTeq (F) (MOI = 0.01) in the presence of trypsin (0.5 μg/ml) for 96 h (rVSV-ZEBOV for 48 h). Viral titers were determined by FFU assays. Data represent the average of three experiments; error bars indicate SEM (one-way ANOVA with Tukey’s post hoc test; ****P < 0.0001). Without indication, P > 0.05, not significant.

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