Fig. 3: Western blot analysis of recombinant glycoconjugate production in E. coli under different media conditions.
From: Recombinant production platform for Group A Streptococcus glycoconjugate vaccines
E. coli cells (ΔwaaL with chromosomally integrated OTase pglB gene) were transformed with A the Shi1 gene cluster, the RhaPS cluster (ΔB) and NanA carrier protein and B Shi1, ΔB and ExoA-10 tag carrier protein plasmids. Cells were tested for glycoconjugate production in different growth conditions. Total cell lysates were run over SDS-PAGE and analysed via His and GAC-antibodies (red and green, respectively). Glycoconjugate production levels vary between the media conditions. Un uninduced, -pglB cells lack the chromosomal pglB. Arrows indicate glycosylated protein bands.
