Fig. 5: Assessment of antibody titers and cytokine levels in mice post-Immunisation studies.

A, B Mouse immunisation study (n = 10) (70 days). Mouse sera were investigated for IgG antibodies triggered post immunisation with the low RhaPS-containing NanA-RhaPS, NanA alone and with low RhaPS containing IdeS-RhaPS. Evaluation of vaccine candidate specific antibodies in mice sera using an ELISA based assay with respective glycoconjugate vaccine candidates showed that IgG levels are elevated in all immunised mice. Y-axis: 450 nm. Statistical analysis was performed using ANOVA followed by a Dunn’s post hoc test. *p < 0.01; **P < 0.001; ****P < 0.0001; ns- not significant. C Pooled mice sera (n = 10) from IdeS-RhaPS immunisation studies were tested in an IdeS neutralisation assay. A series of dilutions from 1:6 to 1:180 was tested in triplicate and the neutralisation activity is displayed in % compared with a no-sera control. * indicates duplicate data obtained for D35. Dashed line, positive control sera; solid line, negative control sera. D, E Immunised mice splenocytes were investigated for IL-17A and IFN-γ levels after restimulation with the mitogens NanA-RhaPS. Statistical analysis was performed using ANOVA followed by a Bonferroni’s post hoc test. *P < 0.05; **P < 0.001; ****P < 0.0001.