Extended Data Fig. 1: Dual colour autofluorescence visualization.

xy image planes acquired by MediSCAPE in fresh mouse brain cortex, including a prominent blood vessel. Contrast corresponds to autofluorescence excited by 488 nm light. Dual colour emission images are acquired simultaneously using an image splitter in front of the camera and positioning each colour channels side-by-side on the camera chip (along y). a and b show grayscale raw emission channels acquired with 525/50 nm and 618/50 nm bandpass filters respectively. These channels are converted to c, ‘yellow hot’ and d, blue colormaps and then merged, as shown in e. This colour-scheme was chosen to enable the highly overlapping colour channels to be more easily distinguished when merged than conventional RBG scaling, while also being more colour-blind accessible. Scale bars, 100 µm.