Extended Data Fig. 1: In vitro delivery of COL1A1 mRNA-containing EVs.
From: Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy
a, Fluorescence images of serum-starved nHDFs treated with COL1A1-GFP EVs and protein translated from delivered COL1A1-GFP mRNA after 48 h. Scale bar, 100 µm. b, Fluorescence intensity of cells treated with COL1A1-EVs (n = 3 for all groups, ***P < 0.001 Control vs COL1A1-EVs) in 48 h. c, RT-qPCR shows higher collagen mRNA transcript levels after in vitro delivery of COL1A1 mRNA from EVs (n = 3 for all groups, ***P < 0.001 Control vs COL1A1-EVs) in 48 h. d, Western blots show elevated COL1A1 protein in treated fibroblasts (n = 3 for all groups, **P = 0.001 Control vs COL1A1-EVs).e, Pro-collagen I collected from supernatant and detected by ELISA (n = 3 for all groups, ***P < 0.001 Control vs COL1A1-EVs) in 48 h. All data are from three independent experiments and are presented as means ± SEM; two-sided Student’s t tests were used for the comparisons in (b–e).
