Extended Data Fig. 2: Characterizing immune responses of glial cells and immune cells on the lung–brain MPS. | Nature Biomedical Engineering

Extended Data Fig. 2: Characterizing immune responses of glial cells and immune cells on the lung–brain MPS.

From: Blood–brain barrier injury and neuroinflammation induced by SARS-CoV-2 in a lung–brain microphysiological system

Extended Data Fig. 2

a, Confocal micrographs of brain endothelial cells immunostained for VE-cadherin and ZO-1 without astrocytes (Δ astrocytes) or without microglia (Δ microglia) following conditioned medium treatment on Day 4 (n = 4). b, Quantification of endothelial cell density based on a. Data are presented as the mean ± SEM and were analyzed using a one-way ANOVA with the Bonferroni post hoc test. c, Confocal micrographs of microglia immunostained for CD206 and F4/80 following conditioned medium treatment on Day 4 (n = 3). d, Quantification of CD206 and F4/80 fluorescence intensity for control and SARS-CoV-2 groups based on c. Data are presented as the mean ± SEM and were analyzed using an unpaired Student’s t-test (***: P < 0.001). e, Fluorescent micrographs of PBMCs (pre-stained with Cell Tracker Red dye) attached to the endothelium following conditioned medium treatment on Day 4 (n = 4). f, Quantification of the density of attached PBMCs based on e. Data are presented as the mean ± SEM and were analyzed using an unpaired Student’s t-test.

Back to article page