Extended Data Fig. 4: Sucrose-based hypertonic buffer led to dose-dependent activation of the cGAS-STING pathway and to enhanced cGAS-DNA interactions. | Nature Biomedical Engineering

Extended Data Fig. 4: Sucrose-based hypertonic buffer led to dose-dependent activation of the cGAS-STING pathway and to enhanced cGAS-DNA interactions.

From: Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS–STING pathway

Extended Data Fig. 4

a, Osmotic pressure of B1mix buffer supplemented with sucrose at increased concentrations. b, Transfection efficiency of mouse T cells in sucrose-based hypertonic buffers. c, qPCR analysis of mouse T cells electroporated with sucrose-based hypertonic buffers. d, MST analysis of the in vitro binding capability of cGAS with Cy5 labeled dsDNA in isotonic buffer: B1mix (305 mOsmol/kg), or sucrose-based hypertonic buffers: B1mix + 0.04 M sucrose (352 mOsmol/kg) and B1mix + 0.17 M sucrose (508 mOsmol/kg). e, In vitro production of 2’3’-cGAMP in the indicated buffers. EP program for the figure: DN100. Data represent mean ± SD, n = 3 in a and c; n = 4 in b, d, e from independent experiments. Statistical significance was determined by one-way ANOVA with Dunnett’s multiple comparisons test.

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