Extended Data Fig. 1: Comparison of LNP formulations containing MC3, Lipid 331, ALC-0315 or A18-Iso5-2DC18. | Nature Biomedical Engineering

Extended Data Fig. 1: Comparison of LNP formulations containing MC3, Lipid 331, ALC-0315 or A18-Iso5-2DC18.

From: Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA

Extended Data Fig. 1

a, Transfection of mFFL LNPs in THP-1 cells (50 ng mRNA per well, n = 3). b, Transfection of mFFL LNPs containing either MC3, Lipid 331, ALC-0315, or A18-Iso5-2DC18 (A18) at the i.m. injection site in mice (0.2 mg/kg mRNA, n = 3 biologically independent mice per group). c. Representative IVIS imaging results from IM injection. d. The abundance of residual ionizable lipids at the injection site three days post-injection (n = 5 mice per group). Statistical significance was analysed using a one-way ANOVA with Dunnett’s correction (a) or one-way ANOVA with post-hoc Tukey test (b) or a two-tailed Student’s t-test (d). Data are presented as mean ± SD.

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