Extended Data Fig. 7: MCATS measures dose-response curves for platelet inhibitors.
a–d) Plots of [Aspirin], [Eptifibatide], [7E3], [Ticagrelor] vs MCATS signal for different donors. A dose-response titration of six drug concentrations for each drug for individual donors is measured with MCATS and all measurements were performed in duplicate or triplicate. Mechano-IC50 for each donor was calculated by fitting plot to a standard dose-response function: Signal = Bottom + (Top-Bottom)/(1+([drug]/IC50). Solid line represents the fitting and dashed line represents 95% CI. Representative RICM, duplex rupture fluorescence (red) and zoom-in fluorescence images for one donor with drug concentrations ranging from 0 mM to 10 mM. Scale bar = 10 μm.
