Extended Data Fig. 1: Comparison of surface density as function of linker length.

a) Scheme showing surface tethered activator sequences with different linker lengths. b) Plot of normalized surface density as a function of linker length. All biotinylated activators were incubated on streptavidin modified surfaces for 1hr at 100nM. Surface density was determined using fluorescence microscopy of fluorescently tagged activators and normalized to the activator density without linker. Error bar represents S.E.M from five independent surfaces.