Extended Data Fig. 6: Quality assessment of the genome-wide CRISPR screen. | Nature Biomedical Engineering

Extended Data Fig. 6: Quality assessment of the genome-wide CRISPR screen.

From: High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2

Extended Data Fig. 6

a) Distribution of sgRNA reads in the plasmid pool extracted from E. coli and unmixed A549-ACE2-Cas9-GFP1-10-sgRNA cell pool at 14-day post-infection. 99.8% and 98.4% of all expected sgRNAs were obtained in the plasmid and cell pools, respectively. b) High reproducibility of sgRNA representations was detected between two biological replicates before (that is, unmixed) and after (that is, unfused) selection. R is the Pearson correlation coefficient. c) Correlation of -log10(negative RRA score) generated by MAGeCK and JACKS score by comparing sgRNA abundance in library-infected A549-ACE2-Cas9-GFP1-10-sgRNA cells (14-day post-infection) and the plasmid pool. Known essential genes are labeled in red. d-e) GO enrichment analysis (d) and KEGG classification enrichment analysis (e) of the depleted genes in A549-ACE2-Cas9-GFP1-10-sgRNA cells at 14-day post-infection.

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