Extended Data Fig. 6: Zip2R and Zip7R augment EphA2-CAR T cell antitumor activity in vitro. | Nature Biomedical Engineering

Extended Data Fig. 6: Zip2R and Zip7R augment EphA2-CAR T cell antitumor activity in vitro.

From: Modular chimeric cytokine receptors with leucine zippers enhance the antitumour activity of CAR T cells via JAK/STAT signalling

Extended Data Fig. 6

a, Transduction efficiency of EphA2-CAR, EphA2-CAR.Zip2R, and EphA2-CAR.Zip7R T cells (N = 3, mean ± SD). b, Frequency of CD4+ and CD8+ T cells transduced with indicated constructs (N = 3, mean ± SD). c, Immunophenotype of CD4+ (left) or CD8+ (right) T cells with indicated constructs (TN-Like: CCR7+ CD45RA+, TEM: CCR7- CD45RA−, TCM: CCR7+ CD45RA−, TEMRA: CCR7− CD45RA+, N = 3, mean ± SD). d, Fold expansion of ΔCAR (left) or CAR (right) T cells stimulated with A673 cells every 7 days (N = 3, mean ± SD). e, Rounds of stimulation (left) and relative expansion compared to CAR (right) in repeat stimulation assays with A673 cells (N = 3, mean ± SD, **p = 0.0011, ****p < 0.0001, two-way ANOVA with Tukey’s multiple comparisons test).

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