Extended Data Fig. 8: Analysis of B7−H3-CAR.Zip7R T cell toxicity in vivo. | Nature Biomedical Engineering

Extended Data Fig. 8: Analysis of B7−H3-CAR.Zip7R T cell toxicity in vivo.

From: Modular chimeric cytokine receptors with leucine zippers enhance the antitumour activity of CAR T cells via JAK/STAT signalling

Extended Data Fig. 8

a-d, Mice received a single i.v. dose of 3x105 B7-H3.CAR.Zip7R.ffLuc T cells on day 7 post A549 cell injection; non-tumor bearing mice served as a control (N = 5 per group). (a) Serial bioluminescence images. b, Quantification of bioluminescence. c, Kaplan-Meier survival (N = 5). d, Representative IHC of lung in non-tumor bearing mice treated with CAR.Zip7R T cells at day 48 post T cell injection. e, Kaplan-Meier survival of A549-tumor bearing mice post i.v. injection of 3x105 B7-H3-CAR.Zip7R control (AAVS1ko) or T cell receptor (TRACko) KO T cells (N = 5). f, A549 bearing mice received CAR.Zip7R.ffLuc T cells and on day 7 ruxolitinib was started (shaded area) in ½ of the mice (untreated: n = 5; treated: n = 5). Quantification of CAR.Zip7R.ffLuc T cell bioluminescence in untreated or ruxolitinib chow-treated mice. g, Fold expansion on day 7 post start of ruxolitinb treatment (N = 4 (untreated), N = 5 (Ruxolitinib), mean ± SD, *p = 0.0159, two-tailed Mann-Whitney U test). h, Kaplan-Meier survival (N = 4 (untreated), N = 5 (Ruxolitinib), **p = 0.0027, log rank test).

Source data

Back to article page