Extended Data Fig. 2: Zip2R(2x) subcellular localization and trafficking determined by confocal microscopy. | Nature Biomedical Engineering

Extended Data Fig. 2: Zip2R(2x) subcellular localization and trafficking determined by confocal microscopy.

From: Modular chimeric cytokine receptors with leucine zippers enhance the antitumour activity of CAR T cells via JAK/STAT signalling

Extended Data Fig. 2

a, Schematic of SNAP/CLIP tag system. b, Representative images of Zip2R(2x) with endosomal markers. Scale bar = 10 µm. c-f, Representative images and Zip2R(2x) colocalization analysis of indicated regions of interest (ROI) with (c) cell membrane, (d) Lamp1, (e) Rab11, and (f) Rab5. g, Comparison of Zip2R(2x) and Zip2R(1x) colocalization with indicated subcellular markers (Membrane: N = 14, Lamp1, Rab11, Rab5: N = 4, *p = 0.0224 (membrane), *p = 0.0479 (Lamp1), mean ± SD, two-tailed t-test).

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