Extended Data Fig. 3: ZipR signaling is inhibited by ruxolitinib. | Nature Biomedical Engineering

Extended Data Fig. 3: ZipR signaling is inhibited by ruxolitinib.

From: Modular chimeric cytokine receptors with leucine zippers enhance the antitumour activity of CAR T cells via JAK/STAT signalling

Extended Data Fig. 3

a, pSTAT5 expression in Zip2R(2x) transduced T cells treated with increasing concentrations of ruxolitinib. IC50 is indicated with a dashed line (N = 2). b, pSTAT5 expression in Zip2R(2x) transduced T cells at baseline or following 24-hour incubation with 5 µM ruxolitinib (N = 4, mean ± SD, ***p = 0.0005, two-tailed t-test). c, pSTAT5 expression in Zip7R(2x) transduced T cells treated with increasing concentrations of ruxolitinib. IC50 is indicated with a dashed line (N = 3, mean ± SD). d, pSTAT5 expression in Zip7R(2x) transduced T cells at baseline or following 24-hour incubation with 5 µM ruxolitinib (N = 5, mean ± SD, ***p < 0.001, two-tailed t-test). e, Viability of untreated or 5 µM ruxolitinib-treated T cells after 7 days of cytokine starvation as determined by flow cytometry (Viability dye/Annexin V) (N = 3, mean ± SD, ****p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons test).

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