Supplementary Figure 4: YBX1 regulates mRNA stability through recruiting ELAVL1.
From: 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs
a, Western blotting showing the NSUN2 and YBX1 knockdown efficiency and Myc-ELAVL1 overexpression in NSUN2 or YBX1 knockdown HeLa cells. ACTIN was used as loading control (n = 3 independent experiments). b, PAR-CLIP assay of RNAs pulled down by Myc-ELAVL1 in NSUN2 or YBX1 deficient T24 cells (n = 2 independent experiments). c, Western blotting displaying the NSUN2 and YBX1 knockdown efficiency and Myc-ELAVL1 overexpression in NSUN2 or YBX1 knockdown T24 cells. ACTIN was used as loading control (n = 2 independent experiments). d, Western blotting showing the levels of YBX1, Flag-YBX1, siYBX1-insensitive Flag-YBX1-WT and Flag-YBX1-W65A in control and YBX1 knockdown HeLa cells used for PAR-CLIP. ACTIN was used as loading control (n = 4 independent experiments). e, Overlap of m5C-containing genes with YBX1 and ELAVL1 binding targets. Unprocessed gels for a-d are provided in Supplementary Figs. 8–10.
