Extended Data Fig. 8: Compartment- and aetiology-specific alterations of cardiomyocytes in heart failure.
a,b, Monocle analyses showing the ordering of CMs along pseudotime marked by heart condition (a) or CM state (b). c, Distribution of CMs from different heart conditions (N_CM, cHF_CM, and dHF_CM) in each defined CM state (Normal, cHF, dHF) based on a. d, Heatmap of different blocks of DEGs along the pseudotime trajectory. Right, representative genes in each gene cluster. For numerical source data, see Source Data Extended Data Fig. 8. e, Selected top GO terms related to corresponding DEGs in d. f-h, Co-staining of ACTN2 and S100A6 (f), DKK3 (g), or ROCK (h) in heart sections from normal, cHF and dHF hearts, respectively. Scale bar = 25 μm. Data are representative of 6 independent experiments yielding similar results. i, Regulon activities of NR1H2, KLF4, XBP1, CREB5, EGR1, and JUN, in normal, cHF, and dHF. Each box represents the median and the lower and upper quantiles, and the whiskers indicate 1.5 times of the interquartile range. Normal LV: n=400 cells, cHF_LV: n=429 cells and dHF_LV: n=305 cells. Two-tailed Wilcoxon rank sum test was used.
