Extended Data Fig. 3: Gli1 Lin + mesenchyme expands distally in fibrotic repair.
(a) Lack of Gli1 Lin+ cells in corn oil-treated Gli1creERT2:R26YFP lung (2 animals). (b, c) Comparison of Gli1 Lin+ cell localization with Gli1 + cells in Gli1LacZ/+ lung. These experiments were each repeated three times independently with similar results. (d, e) Extended washout after tamoxifen (>4wks) followed by bleomycin demonstrates similar expansion of Gli1 Lin+ cells into the alveoli. (n = 3 per group; each data point represents one animal; one-tailed unpaired Student’s t- test). Data are expressed as mean ± SD. (f, g) Deletion of Gli1 + cells does not alter airway epithelial composition during normal homeostasis. (n = 3 per group; each data point represents one animal; one-tailed unpaired Student’s t-test). Data are expressed as mean ± SD. (h–j) Gli1-deleted lungs treated with bleomycin demonstrate reduced expression of Gli1 along with changes in fibrotic markers (n = 6 for control, n = 5 for Gli1- deleted; each data point represents one animal; for (i), Col1a1 P = 0.0862; Snai1 P = 0.3126; Cdh2 P = 0.0310; one-tailed unpaired Student’s t-test for (h–j)). Data are expressed as mean ± SD. (k) Weight loss after bleomycin injury in control and Gli1- deleted animals (n = 6 for control, n = 5 for Gli1-deleted; each data point represents one animal; one-tailed unpaired Student’s t-test). Data are expressed as mean ± SD. AW = airway. Scale bars, 100 μm.
