Extended Data Fig. 3: Inducing filopodia growth by doxycycline in a stable Cos7 cell line.
From: Optogenetic manipulation of cellular communication using engineered myosin motors
a, A Cos7 cell line stably expressing mKate2-ATV+ and Espin1-sfGFP (linked with a T2A linker) under a TetOn-3G system was generated. b, The cells were imaged with or without 24 hours of 2 µg/ml doxycycline induction. Filopodia growth was only observed when doxycycline was applied. Representative images chosen from 3 independent experiments. Scale, 20 µm. pmBFP was used to visualize full cell morphology. c, Quantification of the filopodia density of each individual cell (N = 10 cells each). Statistics determined by two-tailed Mann-Whitney test. The bar graph displayed as mean ± SEM.
