Extended Data Fig. 5: GBP-GFP interaction is necessary for preserving native function of the transported cargo in live cells.
From: Optogenetic manipulation of cellular communication using engineered myosin motors
a, Expression of mKate2-ATV+ & sfGFP-mDia2M/A independently or the covalently linked cargo-motor in Cos7 cells. The prior is based on the binding interaction of GBP-GFP while the latter is based on covalent linkage. GBP-GFP interaction preserved the function of mDia2M/A in generating filopodia, while covalently linkage disrupted cargo function and cells formed fewer filopodia. The white dashed line delineates the smooth cell border. Representative images chosen from 3 independent experiments. Scale, 20 µm. b, Quantification of the filopodia density of each individual cell (N = 10 cells each). Statistics determined by two-tailed Mann-Whitney test. The bar graph displayed as mean ± SEM.
