Extended Data Fig. 2: Bidirectional transport of bulky transmembrane SHH receptors within filopodia using ATVs in live cells.
From: Optogenetic manipulation of cellular communication using engineered myosin motors
a, Expression of sPtch1-eGFP with or without co-expression of mKate2-ATV+ in C3H/10T1/2 cells. Representative images chosen from 3 independent experiments. Scale, 20 µm. b, Quantification of the relative concentration of sPtch1-eGFP at the tip compared to the cell body (N = 33 and 41 filopodia each), based on images from (a). c, Expression of Disp1-eGFP with or without co-expression of mKate2-ATV- in C3H/10T1/2 cells. Representative images chosen from 3 independent experiments. Scale, 20 µm. d, Quantification of the relative concentration of Disp1-eGFP at the tip compared to the cell body (N = 86 and 96 filopodia each), based on images from (c). Statistics determined by two-tailed Mann-Whitney test. Both bar graphs displayed as mean ± SEM.
