Extended Data Fig. 1: Generation of the Drosophila cancer anorexia model.
a, Drosophila cancer model was established by overexpressing a wild-type (ykiWT) and a constitutively active form of yki (ykiS168A) using GMR-Gal4. Expression of the wild-type of yki (GMR > ykiWT) caused the mild rough eye phenotype, whereas the active form (GMR > ykiSA) showed dramatic protrusion of the eye. Scale bars are 200μm. b, The 10 day-old yki overexpression flies showed decreased food intake compared with that of the control in the blue dye feeding assay. n = 3 biologically independent experiments; 1.5 days *P = 0.022, 2 days **P = 0.003. c, UAS-ykiWT and UAS-ykiSA control flies showed normal feeding. n = 3 biologically independent experiments. d, The triglyceride levels of GMR > ykiSA was analyzed with female flies. n = 3 biologically independent experiments; day 10 *P = 0.038, day 15 *P = 0.035, day 20 *P = 0.012. e, Quantification of ovary size in Fig. 1c. n = 3 biologically independent experiments; day 10 ***P = 0.0006, day 15 **P = 0.0014, day 20 ***P < 0.001. f, The thoracic muscle of 15-day-old GMR > ykiSA showed abnormal mitochondrial structure and irregular packing of mitochondria between muscle fibers. n = 3 biologically independent experiments. Scale bar is 10μm. Data are presented as the mean ± s.e.m. Statistical significance was determined with two-tailed Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001. Statistical source data.
