Extended Data Fig. 2: SMAD4 nonstop extension mutations suppress protein expression and function. | Nature Cell Biology

Extended Data Fig. 2: SMAD4 nonstop extension mutations suppress protein expression and function.

From: A pan-cancer analysis reveals nonstop extension mutations causing SMAD4 tumour suppressor degradation

Extended Data Fig. 2

a-c, SMAD4 protein (a: western blot, b: quantification) and mRNA (c) expression after lentiviral transduction of EV, WT and mutants and selection of SW620 cells (n=3 biologically independent experiments). d-e, Expression of the SMAD4 targets PAI (d) and ADAM19 (e) quantified by RT-qPCR in transiently transfected BxPC3 cells treated with 10 ng/ml TGFβ (n=4 biologically independent experiments). f, SMAD4 transcript levels quantified by RT-qPCR (n=3 biologically independent experiments) for the T2A element fusion experiment presented in the main figure panels Fig. 3h–j. g, EYFP and Neomycin (Neo) mRNA levels were quantified by RT-qPCR after transfection of BxPC3 cells (n=3 biologically independent experiments). All panels: mean ± SEM, ANOVA with ***p<0.001, **p<0.01, *p<0.05. Numerical source data and unprocessed blots are provided with the paper.

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