Extended Data Fig. 10: DCP1 inhibits full-length elongation of Tsix transcript.
From: Decapping enzyme 1A breaks X-chromosome symmetry by controlling Tsix elongation and RNA turnover
a, PROseq analysis of Tsix and Xist transcription in differentiating Tsix+/- female hybrid ES cells3. Undifferentiated ES cells (day 0) and differentiating cells (day 3 and day 7) are shown. Strand-specific analysis: Watson strand (+, red) and Crick strand (-, blue). Dashed line indicates region magnified in Extended Data Fig. 8d. Biological replicate 1. b, Shown at an FPM scale of 0-1250, PROseq analysis of Tsix and Xist transcription in differentiating Tsix+/- female hybrid ES cells. Undifferentiated ES cells (day 0) and differentiating cells (day 3 and day 7) are shown. Strand-specific analysis: Watson strand (+, red) and Crick strand (-, blue). Two biological replicates are shown. c, Shown at an FPM scale of 0-200, PROseq analysis of Tsix and Xist transcription in differentiating Tsix+/- female hybrid ES cells. Undifferentiated ES cells (day 0) and differentiating cells (day 3 and day 7) are shown. Strand-specific analysis: Watson strand (+, red) and Crick strand (-, blue). Two biological replicates are shown. d, PROseq analysis of Tsix and Xist transcription in differentiating day 7 Tsix+/- female hybrid ES cells. Allele-specific analysis: Xa (cas allele, light blue), Xi (mus allele, pink). Strand-specific analysis: Watson strand (+, Tsix) and Crick strand (-, Xist). Biological replicate 1. e, PROseq analysis of Tsix and Xist transcription in differentiating Tsix+/- female hybrid ES cells. Differentiating cells (day 3 and day 7) are shown. Allele-specific analysis: Xa (cas allele, light blue), Xi (mus allele, pink). Strand-specific analysis: Watson strand (+, Tsix) and Crick strand (-, Xist). Biological replicate 2.
