Extended Data Fig. 3: p53 is ufmylated at Lys351, 357, 370 and 373 of C-terminal region.
From: UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination
a, The scheme diagram of p53-WT and its mutants. The Lys residues in p53 were replaced by Arg at the indicated positions. b, The ufmylation assay was performed to identity the ufmylation region of p53 in HEK293T cells expressed with p53-WT and its mutants as indicated in (a). c, Lys residues were replaced by Arg at indicated positions, and the ufmylation assay was performed in HEK293T cells. d, All Lys residues were replaced by Arg in p53 except one each of the five residues Lys351, 357, 370, 373 or 382 as indicated, and the ufmylation assay was performed in HEK293T cells. Source data are available online.
