Extended Data Fig. 5: Effect of myosin inhibition on organoid cell shape.
a-b, 3D segmentation of a crypt on 15 kPa gels under baseline conditions (a) and the same crypt after 3 h treatment with 15 µM of blebbistatin (b). Top: medial view. Bottom: lateral view. Representative images of 2 independent experiments. Scale bar, 20 µm. c-d, Apicobasal tilt (c) and basal aspect ratio (d) as a function of the distance to crypt centre on rigid substrates (15 kPa) before and after blebbistatin. Vertical dashed line indicates the boundary between the stem cell compartment and the transit amplifying zone. From centre to edge bins, n = 42, 72, 150 and 232 cells for baseline crypt and 39, 82, 131 and 209 for blebbistatin treatment. Data from 2 independent experiments.
