Extended Data Fig. 4: Trapped PARP1 is modified in a PIAS4-dependent manner.
From: The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin
a. PARP1 SUMOylation by SUMO1 was detected as described in Fig. 3a. Data shown represent 2 biological replicas. b. Western blotting for total ubiquitin input for Fig. 3a. Data shown represent 2 biological replicas. c. A quantification of the SUMO2/3ylated and ubiquitylated PARP1 isoforms in the gels in Fig. 3a. d. PARP1 SUMOylation by SUMO1 detected as described in Fig. 3b. Data shown represent 2 biological replicas. e. PARP1 SUMOylation by SUMO1 was detected as described in Fig. 3d. n = 1 biological replicas. f. Western blotting for total ubiquitin input for Fig. 3d. Data shown represent 2 biological replicas. g. A quantification of the SUMO2/3ylated and ubiquitylated PARP1 isoforms in the gels in Fig. 3d. h. PARP1 SUMOylation by SUMO1 detected as described in Fig. 3e. Data shown represent 2 biological replicas.
