Extended Data Fig. 6: Transcriptional Characterization of PD-1+ LSCs in Notch1-induced T-ALL and Pten null T-ALL.
a,b, Heatmap and expression score for indicated gene sets in PD-1– and PD-1+ T-ALL cells (n = 3 mice). Leukemia stem cell (LSC), acute myeloid leukemia (AML), and acute lymphoid leukemia (ALL). c, Schematic depicting strategy for the transcriptional analysis for PD-1+ and PD-1– leukemic cells from Pten null T-ALL mice. d, GO term analysis of PD-1+ T-ALL cells of differentially expressed genes in PD-1– and PD-1+ Pten null T-ALL cells (n = 3 mice). e, Heatmap of differentially expressed genes in PD-1– and PD-1+ Pten null T-ALL cells (n = 3 mice). f,g, GSEA (top) and analysis (bottom) of the cell cycle (f) and ROS (g) of PD-1– and PD-1+ Pten null T-ALL cells (n = 3 mice). h-j, GSEA of the apoptotic pathway (h), early T lymphocyte genes, MYC pathway, and MYC upregulated genes (i), and NOTCH1 signal pathway (j) in PD-1– and PD-1+ Pten null T-ALL cells (n = 3 mice). k,l, Heatmap of differentially expressed genes and expression score for LSC genes in AML (k) and chemoresistant genes in ALL (l) in PD-1– and PD-1+ Pten null T-ALL cells (n = 3 mice). The P values in a(right),b (right),f(bottom),k(right),l(right) were determined by the unpaired two-tailed Student’s t-test. All data are shown as mean ± s.d.
