Extended Data Fig. 7: Transcriptional Characterization of PD-1⁺ LSCs in human T-ALL.

a, Schematic depicting strategy for the transcriptional analysis for PD-1⁺ and PD-1^– leukemic cells from human T-ALL. b, GO term analysis of PD-1⁺ T-ALL cells of differentially expressed genes in PD-1^– and PD-1⁺ human T-ALL cells. (n = 3 individual patients). c, Heatmap of differentially expressed genes in PD-1^– and PD-1⁺ human T-ALL cells (n = 3 individual patients). d,e, GSEA (top) and analysis (bottom) of cell cycle (d) and ROS (e) of PD-1^– and PD-1⁺ human T-ALL cells (n = 3 individual patients). f-h, GSEA of the apoptotic pathway (f), early T lymphocyte genes, MYC pathway, and MYC upregulated genes (g), and NOTCH1 signal pathway (h) in PD-1^– and PD-1⁺ human T-ALL cells (n = 3 individual patients). i,j, Heatmap of differentially expressed genes and expression score for leukemia stem cell (LSC) genes in acute myeloid leukemia (AML) (i) and chemoresistant genes in acute lymphoid leukemia (ALL) (j) in PD-1^– and PD-1⁺ human T-ALL cells (n = 3 individual patients). The P values in d(bottom),i(right),j(right), were determined by the unpaired two-tailed Student’s t-test. All data are shown as mean ± s.d.

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