Extended Data Fig. 2: Characterization of Pd1-2A-CreERT2; TdTomato reporter mice.
a, Schematic diagram of Pd1-2A-CreERT2 mouse line. The homologous recombination PCR results of F1 generation heterozygous mice. The numbers indicated individual mice; wt, wild type; M, 1 kb DNA ladder. b, Schematic diagram and the Southern Blot result of homologous recombination. The numbers indicated individual mice. c, Schematic diagram of subcutaneous transplantation of B16 melanoma cells into Pd-1-CreERT2; tdTomato or Pd-1–/– mice. d,e, Six weeks after injection, the tumor tissues were digested and FACS analyzed at one day after tamoxifen injection for Pd-1-CreERT2; tdTomato mice (d) and Pd-1–/– mice (e). Plots are representative of n = 3 biological repeats. f, Schematic depicting strategy of T cells with anti-CD3/CD28 antibodies and 4-hydroxytamoxifen for 72 h under in vitro culture conditions. g,h, Representative FACS plots of PD-1 and Tomato expression in CD3+ T cells from Pd-1-CreERT2; tdTomato mice (g) or Pd-1–/– mice (h) with indicated treatments. Plots are representative of n = 3 independent repeats. i, Quantification of GFP+ T-ALL cells in peripheral blood at indicated time points after transplantation. (n = 5 mice). j, The survival curves of T-ALL mice (n = 5 mice). k, Representative H&E staining images for the spleen, liver, and lung sections from control mice or T-ALL mice at the indicated weeks after transplantation. Scale bar 50 µm. l, Representative FACS plots of PD-1 expression in GFP+ T-ALL cells in peripheral blood of T-ALL mice at d42 after transplantation. k,l, Images and plots are representative of n = 5 mice. All data are shown as mean ± s.d.
