Extended Data Fig. 4: Impact of SARS-CoV-2 proteins on CHK1 and RRM2 expression.
A) Immunoblotting of whole cell lysates from Huh7 cells transfected with plasmids encoding for the indicated SARS-CoV-2 proteins and stained for CHK1 and RRM2. Predominant bands at the expected molecular weights are indicated by red arrows. B) Quantification of CHK1 and RRM2 protein levels shown in A; values are the means of two independent experiments, except for NSP13 and ORF6 (n = 4); protein levels are normalized to either vinculin or tubulin protein amounts (house-keeper, HK) and shown as relative to the control-sample expressing GFP. Arrows indicate the viral proteins that most affected both CHK1 and RRM2 levels. Huh7 cells did not express detectable levels of the following viral proteins: NSP4, NSP11, ORF9c, ORF10, M and therefore CHK1 and RRM2 protein levels where not quantified. Source numerical data and unprocessed blots are available in source data.
