Fig. 2: Evidence of ‘direct encapsulation’ of STING by live-cell imaging.

a–c, Sting^−/−MEFs stably expressing mRuby3-STING and Lamp1-EGFP were imaged by Airyscan super-resolution microscopy every 0.4 s from 3 h after DMXAA stimulation (related to Extended Data Fig. 4b–d): the perinuclear region of cell (a); the time-lapse images of the region outlined by the yellow box in a shown sequentially (b); the schematic corresponding to the individual time-lapse images (c). The yellow arrows indicate a cytosolic STING chunk in close proximity to the limiting membrane of Lamp1⁺. A cytosolic STING chunk is depicted as the cluster of vesicles (see also Fig. 3). The cyan arrows indicate STING inside Lamp1⁺. Scale bars, 500 nm (a–c).